Following the same, reliable blood testing protocol, will smooth out anomalies and ensure you’re looking at a true baseline rather than a fluke day.

⏰ Time of Day

- Test in the morning, ideally between 7:00 AM and 9:00 AM.

- Stick to exact time for all blood tests now & in the future.

- Most hormones, like testosterone and cortisol, follow a diurnal rhythm, morning testing gives consistent high-signal data or follows your unique morning pattern into the future.

🍽️ Fasting

- 12-hour fast (only water)

- No food, caffeine, alcohol, or supplements during that time

- Fasting helps stabilise insulin, glucose, lipids, and inflammatory markers

💊 No Supplements

- Avoid taking any supplements for 24–48 hours, especially those that can affect: Liver enzymes, Kidneys, Inflammation, Hormone levels

☕ No Caffeine or Alcohol

- Avoid caffeine for at least 12–24 hours

- No alcohol for 48–72 hours before the test

😴 Normal Sleep

- Get consistent sleep (7–9 hours) for at least 2 nights before testing

- Poor sleep can influence cortisol, insulin, glucose, and inflammation

🌿Calm Morning

- No acute stressors before the test (e.g. arguments, rushing, stressful meetings)

- Stress increases cortisol, glucose, and inflammatory cytokines

🏃 Exercise Protocol

- Exercise elevates markers like CK, lactate, inflammation, and can skew hormone levels

Type of Training & When to Stop Before Testing

- Leg Day (squats, deadlifts, lunges, etc.)

❌ At least 3–4 days before

- Upper Body Training

❌ 2 days before

- Cardio / Conditioning

❌ 1–2 days before (especially if intense or prolonged) |

- Light walking or stretching

✅ Allowed — doesn’t interfere